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Nitrates (NO3)


Nitrates (NO3-) are a chemical compounds made from the elements nitrogen and oxygen. Nitrogen is needed by all plants and animals in order to make proteins (the building blocks of cells), to grow, and to reproduce.

Nitrogen is very common and found in many forms in the environment. Nitrogen is most abundant in its molecular form (N2-) which makes up 79 percent of the air we breathe. In this form, nitrogen is useless to most plants and animals. Blue-green bacteria, the most common algae found in algae blooms, can convert nitrogen (N2-) into other compounds such as ammonia (NH3) and nitrates (NO3-) which can be used by plants in order grow. Nitrogen is a common ingredient found in most fertilizers.

Animals get the nitrogen they need by eating plants or by eating animals that have eaten plants. Animal digestive systems break down plant proteins to form nitrogen compounds which can be used to form the proteins they need. Much nitrogen is released in the wastes produced by animals. Ducks and geese contribute a heavy load of nitrogen (from excrement) in areas where they are plentiful.

When plants and animals die, proteins are broken down by bacteria forming ammonia (NH3). Ammonia is broken down by other bacteria to form nitrite (NO2-). Ammonia and nitrite are both poisonous to animals. Nitrite is then consumed by a third type of bacteria which forms nitrates (NO3-). Nitrates can then be used by plants in order to grow. This recycling of nitrogen through the environment is called the nitrogen cycle.

Nitrate is a major ingredient in most fertilizers. When it rains, varying amounts of nitrates wash from farmlands and lawns into nearby streams. Nitrates can also enter streams from animal wastes, leaking septic systems, and sewage.

Nitrate (NO3-) can be harmful to humans. It is broken down in our intestines becoming nitrite (NO2-). Nitrite affects the ability of red blood cells to carry oxygen. If infants consume water high in nitrite/nitrates, they may suffer from a serious condition called methemoglobinemia or "blue baby" disease. Wells contaminated by sewage or agricultural run off are a major concern in some areas. Nitrites can also cause serious illnesses in fish.

How to Test for Nitrates

Nitrates can be tested for in a variety of ways. Most tests involve a colormetric change. Some kits measure this change through electronic means, others use a simple color wheel comparator. Testing procedures vary for different levels of nitrates. Suspected nitrate nitorgen in the 0 to 1 mg/liter range requires one test while a high range test 0-10 mg/liter Nitrate test is necessary for water with higher nitrate levels.

Listed below are the instructions for measuring nitrate (NO3-) levels between 0-50 mg/l using a Model NI-11 test kit manufactured by Hach Co.

  1. Rinse a color viewing tube several times with the water to be tested; then fill to the 5-ml mark.

  2. Open one NitraVer 5 Nitrate Reagent packet. Add the contents of the packet to the tube containing the water sample to be tested. Stopper the tube and shake it vigorously for exactly one minute. An amber color will develop if nitrate is present.

  3. Allow the prepared sample to set undisturbed for one minute.

  4. Place the amber colored disk into the black comparator box.

  5. After the sample has set undisturbed for one minute, place the tube into the top right opening of the black comparator box.

  6. Fill the other viewing tube to the 5-ml mark with some of the original water sample and place it into the top left opening of the black comparator box.

  7. Hold the comparator box up to a light source and view through the openings in the front. (Use a white sheet of paper for a background.) Rotate the color disk in the comparator box until the two colors match. Read the mg/l nitrate nitrogen (N) through the scale window.

  8. Test results can be converted from mg/l nitrate nitrogen (N) to mg/l nitrate (NO3-) by multiplying the scale reading by 4.4.

  9. When finished, pour the used chemicals into the waste collection container. Clean the equipment and return it to its proper place.

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